FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404310

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404310
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4682654
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG5100.020.9633711
GGTCTAA7350.017.361371
GTATTAG8350.016.521261
GTATTAA14200.016.5154421
GTTCTAG12550.016.4883481
GTCCTAG11150.016.3934611
GTACTAA8850.015.5878551
GTGCTAG11550.015.5271231
GTATAGG7200.015.3280571
GTTCTAA13800.015.2447541
GTCTAGA8150.015.234021
GGTCTAG8250.015.0493651
GTCTTAG10500.014.7806261
TAGACGG3051.4731995E-814.7009575
TTAGGAC7250.013.7961363
GTACAGG21750.013.7952521
GTCTAGG9850.013.6551981
GTTCTAT11650.013.6176311
GCTCTAG14450.013.6043141
GTATATA14200.013.6009521
Produced by FastQC (version 0.11.5)