FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404311

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404311
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4682654
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG10850.019.7623081
GTACTAA8000.018.5889231
GTACTAG5600.018.5271641
GTCTTAG9050.016.4321961
GTACAGG20900.015.7200191
GTGCTAG10800.015.3706861
GTATTAG8550.015.3706851
GTATTAA13150.015.2537981
CGAATCG2051.4533725E-515.1367089
GTACTAT7550.015.1162031
GTATTAC8000.014.6982171
ATAAGCG3202.3213943E-814.20172456
GCCCTAG10200.013.9014281
GCTCTAG14450.013.8814851
CTTAACG2751.0699514E-613.8106893
GATCTAG8550.013.7527181
GTTCTAA12850.013.7259621
GTATAGG7350.013.6454041
GTATAAG8900.013.6004661
GTCTTAA13900.013.4355281
Produced by FastQC (version 0.11.5)