FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404312

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404312
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4633327
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCTAG10600.018.2290631
GTCCTAG11300.018.0158921
GTATATA13550.017.5708181
GTATTAG7200.017.2525081
GTACTAG6100.016.4040241
GTCCTAA9300.016.3249531
GCGCATA1704.959929E-516.2376541
CGCGTCA2351.8213177E-716.14849315
GTATTAA13400.015.1925051
GTCGCGT1450.004095923614.80594565
GTCTATA7650.014.433471
TCAGTAC11500.014.4022483
GCTCTAG13250.014.3228361
GTGCGTA2657.09515E-714.3228351
GTATAAA16150.014.31477451
GTCTTAA11450.014.16363051
TATACTG12800.014.0178135
TAGACGC2506.1077008E-613.8021555
GCGTATG2253.472803E-513.8020051
CTAGTAC5150.013.400153
Produced by FastQC (version 0.11.5)