FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404322

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404322
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15212452
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG37900.016.4406261
GTATTAG28450.015.0111891
CGATCGT6550.014.96260338
GTATTAA46050.014.595171
GTATATA55800.014.2406291
GCGATCG6750.014.00072237
GTTCTAA45100.013.8159591
GTACTAG18150.013.6936511
GTACTAA30200.013.6776861
GTGCTAG36700.013.6397851
GTTCTAG45250.013.3833571
GTCTATA30800.013.18392851
TCTGTAC64850.013.0085943
GTCTTAA41500.012.990031
GTGTTAG39700.012.8735961
GTCTAGA35400.012.8551651
GTACAGG66800.012.83888151
GTCCTAA33550.012.7293091
GTACTAT30150.012.4232151
GTCTTAG36100.012.3149911
Produced by FastQC (version 0.11.5)