FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404323

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404323
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15212452
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG38050.016.7519281
GTTCTAG43300.015.9340591
GTGCTAG36350.015.5120641
GTACTAG19600.015.1883621
GGTCTAA32050.014.6428241
GTATTAG29800.014.2205771
GTACTAT30100.014.1951981
GTTCTAA45850.014.1312471
GTACTAA28450.014.0336521
GTCTTAG32500.014.009031
GGTCTAG28600.013.8375921
GTCCTAA34000.013.8030151
GCTCTAG44800.013.4461681
GTATATG44650.013.17758751
GTGCTAA36150.013.078971
GTATTAA46250.012.9488881
GCCCTAG37950.012.7354821
GTATACG6600.012.7354821
GTATATA54150.012.6766851
GTATTAT45150.012.4886711
Produced by FastQC (version 0.11.5)