FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404326

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404326
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12680321
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG26150.016.4638081
GTACTAG15100.016.4228121
GTACTAA24400.016.0918881
GTATAGG20800.015.89644051
GTATATG36300.015.1811731
GTCCTAG30600.014.9700151
GTATTAA35700.013.8926881
GTATACG5500.013.7769161
GTTCTAG35750.013.584231
GTCCTAA27650.013.2039161
GTGCTAG28900.013.1095211
CTATACT28900.013.1094684
GTATATA49900.012.8382071
GTCTTAG30050.012.6078251
CGAATCG5750.012.60526645
CGATCGT5257.2759576E-1212.46915438
GTACAAG46900.012.4109741
GTTCTAA35050.012.0867371
GTCCTAT24400.011.8571811
GCTCTAG36700.011.8248731
Produced by FastQC (version 0.11.5)