FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404330

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404330
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12624408
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG15650.017.3853821
GTATTAG24000.017.0768261
GTGCTAG30650.015.16962051
GTATTAA35600.014.8984881
GTACTAA22700.014.8686551
GTCCTAG32600.014.7904661
GTTCTAG36000.014.2546061
CGATCGT5200.013.91423938
GTATATA44300.013.682971
GTATACG4609.094947E-1213.4767811
GTCTTAA32750.013.1452891
CAGTACT47700.012.9964744
GTACTAC19350.012.8151281
GTTCTAA36050.012.8017981
GCGATCG5700.012.69248537
GTCTTAG29300.012.4597611
CTAGACT26600.012.4297124
TCTAGAC30900.012.2604293
GTACAAG41650.012.0728361
GTGTTAA35200.011.9368191
Produced by FastQC (version 0.11.5)