FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404331

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404331
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12624408
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG14400.016.988371
GTTCTAG37900.015.545791
GTCCTAA28750.015.4599371
GTATTAG25750.014.85257151
GCTCTAG39700.014.8409431
GTCCTAG30950.014.8063121
CGATCGT4600.014.23288638
GTATAGG19300.014.103461
GTCTTAG29600.014.0847631
GTATTAA34650.014.0207671
GCGATCG5250.013.78195237
GTACAGG53350.013.7562841
GTACTAA24300.013.7537571
GTGCTAG29550.013.2923961
GTTCTAA36900.013.0724671
GTATATA47300.013.0391051
CTATACT31200.012.9147454
TCTAGAC27600.012.7275243
GGTCTAA22900.012.6386211
GTCTTAA35200.012.627081
Produced by FastQC (version 0.11.5)