FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404332

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404332
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12571418
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG13900.016.8500941
GTATTAG25250.015.9599921
GTCCTAG30950.015.691581
GTTCTAG37400.014.6431251
GTATATA47450.014.3000711
TACGCGA2657.2419425E-714.297392
GTGCTAG31050.013.8661741
GTCCTAA27350.013.8529911
GTACTAA24400.013.6927321
GTATTAA35700.013.5072831
TCTAGAC29450.013.4501123
GCGATCG4901.8189894E-1213.358737
GGTCTAA22350.013.2534561
GCCTTAG29850.013.154331
GTCTTAA32800.012.7063181
GTATTAT37400.012.6170321
GTCTATA21750.012.5105391
CTTATAC27600.012.4797033
GTACAGA60200.012.24406051
GCTCTAG35950.012.1678271
Produced by FastQC (version 0.11.5)