FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404336

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404336
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2971878
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG6600.023.4688631
GTATTAG4250.017.8179281
TAGTGCG1452.3819413E-416.95078857
GTATAGG3750.016.5220781
GTGCTAG7300.015.0886551
CGTAGAC2353.2992593E-614.6472333
CTATCCT8550.014.09046654
GCCTATA4403.6379788E-1214.0813171
GTGTATG10150.013.9040481
GTATAGA6050.013.6546111
GTATATG7100.013.5744771
GTGCTAA6900.013.4690871
GCCTTAG6450.013.3414721
CCTAGAC7300.013.2025743
ATTAGAC5250.013.11276153
GTATATA9550.012.9754541
CGAGCGT2153.7364673E-412.80458116
GTTCTAG7350.012.6444481
GTATTAT7400.012.5590121
GTATTAA7200.012.4298061
Produced by FastQC (version 0.11.5)