FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404337

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404337
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2971878
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33300.1120503600753463No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTAA6500.020.7049051
GTCGTAT1852.639954E-718.6530671
GTTCTAA7600.018.1621951
CTAGCGC950.0079268518.1221434
TCGCGCG950.00795149818.1123879
GTATTAG5250.017.0897641
CGAATCG1452.7768844E-416.6134329
GTACTAG2603.2961907E-815.9268491
GTATTAA7450.015.7486961
CCTAGCG1350.003275011415.3119093
GTACTAA4550.015.1684281
TACTAAC4403.6379788E-1214.1055572
GTGCTAG7400.013.98981
GCGTAGT1500.00652179913.8032691
GTACGAA1500.00652179913.8032691
GTATAGA6850.013.6017621
GTCTAGG6400.013.4797551
GTACTGA9000.013.4198461
GTATAGC3901.9153958E-913.2723751
GCTCTAG8350.013.22468951
Produced by FastQC (version 0.11.5)