FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404338

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404338
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3028620
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAT1103.324756E-521.9066471
TAGTGCG1702.0626449E-618.58454957
CTACGCT2255.693437E-918.3604624
GTACTAA5250.017.7041471
GTGCTAG6800.016.1998731
GTATATG8400.015.1632731
GTTCTAG8450.015.0735491
CGTAGAC2101.8511832E-514.75394253
GTACTAG3757.094059E-1114.6878841
TCTGTAC12200.014.6733213
GTATTAG5550.014.2661041
GCTACGC2202.8712619E-514.0833093
GTCCTAG6600.014.0828441
GTATAGA5650.014.0136071
GTCTATA6500.013.7698921
GTTCTAA7800.013.6816241
GTCCAGA18400.013.4705461
TACGCTC2052.4896985E-413.43448455
ATAGTAC4951.8189894E-1213.2139693
GTATTGG7600.013.1357541
Produced by FastQC (version 0.11.5)