FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404339

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404339
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3028620
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAT4200.018.0480821
CTAGACT5900.015.7502954
GTCCTAG7250.015.6831611
CTACTAG3857.2759576E-1215.2140851
GTACTAG3402.4920155E-1015.2009251
GTCCTAA6600.015.1395061
CTCGCGA1400.004198896714.75052120
CGAATCG1400.004200279214.749799
GCTCTAG8450.014.2714791
CTAGGAC6800.014.1734723
GTATTAG5400.014.0373961
CTATACT6400.013.9820344
GTACATA7950.013.8688521
GTCTACG2506.1981737E-613.7821711
CCGCTAA1500.006588237413.7821711
GTACTAA5250.013.782171
GTGCTAG6800.013.6808321
GGTCTAG5800.013.663361
TACTAGA5600.013.5264472
GTCTTAG7400.013.5028031
Produced by FastQC (version 0.11.5)