FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404340

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404340
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2954850
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTGCG901.8969683E-423.39260157
CGCTAGA950.00793076218.120591
CTAAGCG2151.3040535E-616.0140884
GTCCTAA5800.015.4337441
GTATTAG5150.015.3761131
GTCCTAG7700.015.2024691
GTGTTAG7250.015.1963021
ATAGTAC4750.014.4969633
ACGCCTA1709.2621683E-414.1771765
GGTCTAC3901.3096724E-1014.1247661
CAATACG1500.006620110513.7721144
CTAGCGG1500.006620110513.7721143
GTATTAA6600.013.5629861
GGTTAGA5850.013.5362361
GTACTAA4609.094947E-1213.4722641
TCTATAC5700.013.2888833
GTATTGG8300.013.2738781
TATACCC5003.6379788E-1213.08350855
GTCTTAG5053.6379788E-1212.953531
GTTCTAA7900.012.6385371
Produced by FastQC (version 0.11.5)