FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404344

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404344
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10396243
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG10600.016.1842651
CGATCGT4800.015.31614638
GTCCTAG24550.014.9740941
GTGCTAG27550.014.7414151
GTTCTAG27700.014.4088011
GTATTAG18150.014.0815031
GTCCTAA20550.013.970271
GTACTAA16900.013.8800451
GCGATCG5700.013.512053537
GTACAGA42500.013.5100731
ATCTCCG120950.013.33974170
TCTCCGA101650.013.18693170
TACGCGA2701.1668579E-512.9631812
GTACTAT16050.012.8700351
GTATAAG20300.012.7625791
GCTCTAG29450.012.6015321
TTGCGCG5003.092282E-1112.59887812
ATTAGAC18500.012.4866953
CTATACT21400.012.4301164
GTCTTAG20900.012.396191
Produced by FastQC (version 0.11.5)