FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404345

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404345
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10396243
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCGT4700.018.62016538
GCGATCG4450.018.09259237
GTCCTAG25650.016.5125561
GTTCTAG27850.015.8365891
GTCTTAG22900.014.8269741
GTATAGG16550.014.8052691
GTATTAG16400.014.7272451
GTACTAG11650.014.4222041
GTACTAA18350.014.30676651
TCTGACG95650.014.157670
GTCCTAA21050.013.4694291
GGTCTAA19450.013.1377061
GTGCTAG23950.012.8615541
GTTCTAA26350.012.6199971
GCTCTAG30750.012.5217161
GTCCTAT20200.012.4766331
GTATTAA24050.012.3714361
CTAGACT20900.012.2172984
CCTCTAG41000.012.0379231
GTCTTAA23950.011.9846311
Produced by FastQC (version 0.11.5)