FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404346

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404346
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10338784
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCGT4000.017.4997438
GTACTAG11650.016.5291141
GTGCTAG25550.015.3475811
GGTCTAA18200.015.1973721
GTATTAG17550.015.1617481
GTTCTAA26200.013.764131
GCGATCG5100.013.72535237
GTATTAA25500.013.3181641
GTCTTAA25500.013.3181641
GTACTAA16300.013.3173211
GTACAGG47200.013.2777311
GTCCTAG25150.013.2250841
ATCTCCG123200.012.92588170
GTCCTAA20100.012.8898681
TCTCCGA105150.012.68163270
GTCCTAT20350.012.5594681
GTTCTAG29400.012.5041681
GTATAGA22300.012.2462341
GTCTTAG21750.012.2339631
GGTCTAG19450.012.0605751
Produced by FastQC (version 0.11.5)