FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404347

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404347
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10338784
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG12850.016.6157191
GTTCTAG27950.016.0295221
GTCCTAG25350.016.0166781
GTATTAG17700.014.4358391
GCTCTAG30350.014.4159661
TCTGACG98050.013.9584270
GTATTAT26000.013.3276711
GTCTTAG21800.013.3264361
GTATAAA34650.012.8290021
GTTCTAA27300.012.8212321
GTATTAA26100.012.6060711
GTGCATA22000.012.5688881
GTCTTAA24650.012.4956291
GTGCTAG23650.012.4319871
GTCGTAC5751.8189894E-1212.1745981
GTGTATA22750.012.1545291
GTATATA29950.012.1542711
CTGACGC85400.012.13223970
GTATAGG17150.012.0414921
GTGTTAA27050.012.0339471
Produced by FastQC (version 0.11.5)