FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404350

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404350
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3361580
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGCG450.004738461730.5136683
TAATACG1305.142587E-621.125794
GTATATA9350.016.1533341
GTACTAA6100.015.7561211
GTACTAG3402.6375346E-1015.143751
GTCTTAG7050.015.0936281
GTATTAA8600.014.768091
TAGTGCG1450.00453232714.58189957
CGATCGT1657.5559935E-414.56377538
GTATTAG5150.013.9969411
GTCTTAA8400.013.893791
GCCCTAG10200.013.7976381
GCGATCG1500.0067535513.730741537
GTTCTAA8300.013.6476211
GGTCTAG6350.013.5141081
TGTACTG12300.013.3968425
CTAGTCG1550.00839653513.28603659
GTCCTAG8050.013.2186441
GTACTAT6500.013.2022441
GTGCTAG7900.013.0351271
Produced by FastQC (version 0.11.5)