FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404352

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404352
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3325006
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG1004.478245E-420.6020344
GTATTAA6700.017.9368231
GTACGAT1554.694107E-415.5066751
GTATATA8900.015.4320041
GTGCGTA1350.003349237115.2605351
GTACTAC5300.014.9006181
GTTCTAA7850.014.8717331
GTATAGG4950.014.56687451
GTCTTAG7600.014.457351
ATTAGAC6450.014.3735113
TTAGACA9600.014.3069684
CTAGGAC7700.014.2698063
CCCTTAG9150.014.2598461
TCTGTAC14600.013.8757993
GGTCTAG6300.013.6254791
GTCCTAG7100.013.5410391
CCTAACC7150.013.4465483
ACACGAC2609.629866E-613.2064323
ACAGTAC9150.013.1342663
TAGTACC4204.456524E-1013.0806554
Produced by FastQC (version 0.11.5)