FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404353

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404353
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3325006
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG4100.017.6364481
GTATTAG5750.017.3662411
GTCTACG2401.26219675E-817.2165341
GTTCTAA8700.016.2270771
GGTCTAG7450.016.1766071
GGTCTAA6300.015.3035861
CGATTCG1808.4366766E-515.25870921
GGTTATA5250.015.0849631
GTCCTAA7700.014.7570291
GTATAAA10400.014.5678361
ACGTAAA2400.004708529414.49798470
GTCCTAG6950.014.3677551
GTACTAT5050.014.3187021
GTATAAG5850.014.1263871
TAGATAG4950.013.8742675
CTAGGAC7800.013.66268353
GTTTATA8450.013.4472331
TAGGACG2304.4543496E-513.4411714
GTGCTAG7750.013.328931
CCCCTAT5700.013.2899561
Produced by FastQC (version 0.11.5)