FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404354

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404354
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3398151
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCGT1959.913492E-1021.1300638
GTTCTAG9100.018.1082821
GTGCTAG8250.017.0611111
GTACTAG3850.016.9422171
GCGATCG2251.1729935E-716.78443537
GTCCTAG8900.015.4293411
GTATTAG6050.015.3209521
CGAATCG1350.003357050515.2552099
GTATAAA11050.015.2233821
GTCTAGT4900.014.0123611
GTGCATA7200.013.8274761
GGTTTAG8250.013.7321141
GTATTCG2002.067949E-413.7321131
GCGGACT3702.5291047E-713.48465269
GTACATA8700.013.4164311
TTGTACG1550.00838360313.2891424
CTAACGG1550.008386048513.28855536
GTTCTAA7950.012.9548231
GTGTATA6950.012.8429831
GTATTAA8850.012.8011231
Produced by FastQC (version 0.11.5)