FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404356

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404356
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3326133
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCTAA8150.018.1072181
GTATTAG5150.017.9927431
GTCCTAG9650.016.7151951
GTACTAG3800.016.2566011
GTATAGG4750.015.1728281
CTAGACT6650.014.9663954
GTACATA8050.014.9215191
GTATTAA7650.014.8044881
GTCTAGA7450.014.7412591
CGATCGT1400.004271116614.71277938
GCGATCG1400.004274532714.71100837
CTTAACG1951.6726203E-414.0797923
GTGCTAG7600.013.998741
CGACCGT1500.00676312713.72779756
GGATATA7600.013.5471691
GATTTAG7350.013.5410251
GTTCTAG8450.013.402881
CTATACT6150.013.3929734
TTAACGA2052.5559115E-413.3929734
TACGCTA1550.00840098513.28496555
Produced by FastQC (version 0.11.5)