FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404357

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404357
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3326133
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG8750.019.238091
CGATCGT1259.083485E-519.2253638
GTACTAG3650.018.8239651
GTTCTAA8950.018.424351
GTCTTAA7500.018.3219931
GTCGTAA1402.152831E-417.1768671
GCGATCG1603.0833762E-517.16343737
TAGACCG1402.1699686E-417.1590485
GTGTTAG8250.016.2399461
GTCTAGT5100.016.1664641
GGTCTAG6200.015.514591
TAATACG2001.2019731E-515.4431455
GTTCTAG8750.015.3119511
GTCTATA5400.015.2683261
GTTTTAG11800.015.1389341
GTCTTAG5750.014.9364071
GTCTTAT7550.014.5605241
GTCCATA6850.014.5439181
ATACACG1901.3416102E-414.4499434
CTATTAG4550.014.3455151
Produced by FastQC (version 0.11.5)