FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404358

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404358
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11611685
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG19100.018.6987761
CGATCGT4400.016.70687138
GTACTAG12000.016.6318281
GTCTTAG24250.015.882811
GTTCTAG30500.015.842571
GCGATCG5350.014.39477637
GTCCTAA22750.014.005751
GTCCTAG27550.013.980331
GGTCTAA20150.013.727721
GGTCTAG23950.013.5963961
TATACTG29700.013.4378365
CGAATCG5001.8189894E-1213.2981859
GTATTAA27250.013.2347911
GTATAGG16950.013.2207671
GTTCTAA29550.013.1526081
GTGCTAG27200.013.130391
GTATATA32350.013.0965671
GTACTAA19800.013.0861811
ATCTCCG173500.013.0120570
TCTCCGA145750.012.75179970
Produced by FastQC (version 0.11.5)