FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404360

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404360
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11341502
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG12750.018.1243111
GTATTAG20650.017.6336021
GGTCTAG23250.015.0593011
GTCCTAG28150.014.552421
GTACTAT17850.014.51514151
CGAATCG4151.8189894E-1114.335929
GTATAGA23100.014.0960921
GTCCTAA20200.013.5198231
ATCTCCG161600.013.51463770
GTACAGG48250.013.3520591
GCTCTAA29700.013.2035091
GTGTTAG25750.013.0533431
GTTCTAG27150.013.0250461
TCTCCGA135700.012.99905770
GTCTTAA26950.012.8618731
GTATACG4902.1827873E-1112.8618721
GTGCTAG26550.012.7918971
GTATTAA26950.012.6020371
TCTAGAC24500.012.5722263
GGATTAG21650.012.4526161
Produced by FastQC (version 0.11.5)