FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404362

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404362
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11474250
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCGA2201.3962781E-615.9104322
GTATTAA28700.014.2727921
GTGCTAG26900.014.1866271
GTCCTAG26400.014.0574611
ATCTCCG162300.013.67193370
GTATTAG18800.013.594711
CGATCGT4951.8189894E-1213.43436238
GTACTAG10950.013.428891
CTATACT23100.013.3342834
GTATTAT28150.013.3079241
TCTATAC21950.013.2357393
GGTCTAA18400.013.1291381
GTATAGG14700.013.0993671
GCGATCG5950.012.94130937
GTATATA32000.012.9103191
GTTCTAG31500.012.8929521
GTACTAA19850.012.8755951
GTCTTAG25350.012.7061741
TATACTG27500.012.6008965
GGTCTAG22800.012.5916881
Produced by FastQC (version 0.11.5)