FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404365

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404365
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11180299
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG13600.016.726881
GTATTAA26750.014.9149411
GTCCTAG28350.014.5669771
GTATAGG14900.014.3279491
GTACTAA19550.014.1423241
GTATTAG17700.014.0386561
TCTGACG124300.013.91013270
GTACTAC15200.013.5846631
GTATAAG20400.013.5530611
GTTCTAA28000.013.4991431
GTGCTAG25750.013.4554571
GTATATA31450.013.35368251
GTCCTAT20950.013.1972521
GTCCTAA23350.013.1897411
GTGTTAG27500.012.9809941
GCTCTAG31450.012.7972791
ATAGGAC21150.012.5739523
GTCTTAA24300.012.5300681
GTCTTAG20700.012.5112831
GTACTAT18200.012.1146151
Produced by FastQC (version 0.11.5)