FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404366

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404366
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12847045
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG27000.017.966741
GTACTAG15400.016.978791
GTATTAA36000.015.5775441
GTCCTAG32250.015.25528051
GTGCTAG32050.015.02843951
GTACTAA22550.014.494091
GTATAGG20050.013.7274391
TATACTG36700.013.218195
GTCTTAG27300.013.1064351
GTTCTAA35100.013.0364231
GTTCTAG35800.012.9737231
GTACAGG57250.012.86033251
GTGTTAG36150.012.8481141
GTACTAT23650.012.8016351
GTATAAG25850.012.6437811
GGTCTAA24550.012.6126091
GTATATG34700.012.4926611
GACGCAA9000.012.34558851
CTATACT28550.012.2916814
GCTCTAG37650.012.2448571
Produced by FastQC (version 0.11.5)