FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404367

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404367
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12847045
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAA25700.015.5616271
GTCCTAG33200.015.4731361
GTATTAA39850.015.400041
GTATTAG27650.014.8382251
GTACTAA24000.014.6527481
GTCTTAG30500.014.5820991
GTGCTAG33100.014.478281
GTTCTAG37850.014.300921
GGTCTAA24500.014.212991
GTATAGG21950.013.5080941
GTACTAT25350.013.4644121
GTACTAG14550.013.2695191
CTAGTAC13850.013.1768883
GTATATG40100.013.1545871
GTGCTAA31100.013.0813261
GTCCTAC29050.012.8176321
GTATATA47400.012.8016071
GTTCTAA37650.012.72857951
GCTCTAG37800.012.678071
GTACATG47600.012.5305261
Produced by FastQC (version 0.11.5)