FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404370

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404370
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12767578
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAA23150.016.6453321
GTACTAG17150.015.6478961
GTATTAG25550.015.6204141
GTCCTAG33800.014.6578831
GTCTTAG28650.014.6507761
GTACAGG53050.014.1382791
GTATTAA36800.013.7434241
GTCCTAA26300.013.6051411
GCTCTAG37250.013.3003061
GTACTAT23700.013.2104771
CTAAGCG8350.013.1852164
GTATAAG24300.013.0258781
CGAATCG5053.6379788E-1212.9446219
GTGTTAG33500.012.8378011
CTATACT28250.012.7877644
GTTCTAA35400.012.5375271
TCTAGAC30200.012.5316853
GTATATA48800.012.3379691
GTCCTAT27750.012.1503421
GTATAGA29250.011.9977481
Produced by FastQC (version 0.11.5)