FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404371

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404371
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12767578
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG31300.017.9240021
GTACTAG15800.016.3378891
GTATTAG23950.015.8080721
GTCCTAA30500.014.5573251
GTGCTAA30700.014.3503771
TCTATAC27650.014.308713
GCTCTAG34650.014.3037851
CCTATAC19600.014.0420873
GTCTTAA34850.014.0241751
GTTCTAG38050.014.0206711
GTTCTAA36700.013.9737171
CTATACT29950.013.8991984
GTGTTAG33200.013.5807881
GCGATCG4901.8189894E-1213.34453137
GTATATA47900.013.2931521
GTATAGG18600.013.1382381
TATACTG36450.013.0251495
GTACTAA22400.012.9069311
GGTCTAA24350.012.862761
GTCTTAG28150.012.7158871
Produced by FastQC (version 0.11.5)