FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404372

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404372
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12699277
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG34700.016.0588131
GTACTAG16050.014.78774451
GTCCTAA25650.014.7513991
GTATTAG23500.014.6372681
GTTCTAG37100.014.3709561
GTTCTAA37450.014.14481
GTATTAA34950.014.0739741
GTATAGG19250.013.2229671
GTATATA49950.013.2218931
GTGCTAG33000.012.9251521
GTACTAA23050.012.8338041
GTCTTAG28300.012.6408071
CCGATCG3002.9673938E-612.6123489
GTATATG39200.012.5480981
ATAGGAC25050.012.4956793
GCTCTAG35000.012.3831281
GTATAGA29000.012.3356851
GTGCTAA30750.012.3048251
GTACAGG54850.012.2915691
GTGTAGA42950.012.0932121
Produced by FastQC (version 0.11.5)