FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404373

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404373
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12699277
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG16000.018.9244651
GTCCTAG35800.018.0690671
GTATTAG25450.016.4942651
GTGCTAG31650.015.0025911
GTCCTAA27200.014.674051
GTATTAA37500.014.4972871
GTCTTAG29550.014.4386011
CTATACT28900.014.04583554
GTACTAA26000.013.6309071
GTTCTAA36900.013.5208031
TCTAGAC26850.013.4526673
GTTCTAG37700.013.4164271
GCCCTAG34850.013.2300941
TTATACT40750.013.0848124
CGATCGT4751.6370905E-1113.04148638
GTATATA48350.013.0231341
GTATAAG25150.012.8602911
GTACTAT22100.012.7668121
GGTCTAG24000.012.7596771
GCGCTAG5451.8189894E-1212.6268321
Produced by FastQC (version 0.11.5)