FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404375

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404375
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6486241
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG7950.016.496111
GTACTAA11950.016.4616011
GTTCTAG17750.016.3322531
GTATTAG12300.015.9931821
GTCCTAG16700.015.4992221
GTATTAA16800.014.1744071
GGTCTAG13150.013.9096191
GTTCTAA18100.013.7283721
GTATATG17000.013.6016311
GTCTTAA17800.013.5719791
GTCCTAA13450.013.3427761
GTGCTAG17800.012.7964371
CTAGACC12050.012.5759874
GTACTAT11150.012.3808441
GTATATA20950.012.3549891
TATGCAC12850.012.3356593
GTGTTAG16400.012.2053221
GTCGACG2852.1880449E-512.19359851
GCTCTAG20050.012.0489391
GTACAGG27500.012.0476871
Produced by FastQC (version 0.11.5)