FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404380

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404380
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6404865
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG6600.016.1693211
GTATTAG11300.015.5369491
GTACTAA12500.014.8716021
GTACTAT10950.014.776031
GTATAGG9950.014.5311581
GTCCTAG16600.014.3091891
GCCTTAG14650.014.0989781
GTATTAA17750.013.9639451
GTACAGG26200.013.9276741
GGTCTAA11750.013.7700011
GTTCTAG16200.013.1750021
GGTCTAG13900.013.1260811
TTGCGCG2103.0582666E-413.1122412
TAATACG2701.3836565E-512.75014
GTATTGG16200.012.7500021
GCTCTAG18650.012.7363281
ATTAGAC12300.012.5945143
CCCTTAA17600.012.5181831
GTCTAGA14700.012.4117361
CTAGTAC7500.012.3930033
Produced by FastQC (version 0.11.5)