FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404381

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404381
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6404865
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG72860.11375727669513722No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG8050.019.6851981
GTATTAG12550.018.6656481
GTATTAA17450.016.5829471
GTACTAA10800.016.267631
GGTCTAG13450.016.136011
TTGCGCG3301.6189006E-1015.64352212
GTCTTAG14300.015.4177771
CTATACG2705.4151315E-815.2970834
GTTCTAG19100.015.1503891
CCTATAC10400.014.89329053
GTCCTAG16750.014.807971
GCTCTAG17850.014.2814191
GTTCTAA18400.014.2289741
CTAGACT14400.014.1019974
GTATATA19900.013.5026621
TCTATAC12200.013.2601833
GTCGTAA3406.2338586E-813.1717131
GTATAGA15700.012.9458391
GTCCTAA13350.012.9022851
GTATTAT18200.012.3032491
Produced by FastQC (version 0.11.5)