FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404382

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404382
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11269637
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG13050.018.5136281
GTCCTAG28650.016.865821
GTATTAG23250.014.3071661
GGTCTAG21450.014.2018511
GTTCTAG30400.013.9368541
GTCCTAA24600.013.6643531
GTATTAA30550.013.63919451
GTACTAA18900.013.5242761
GTGCTAG29150.013.3332921
ATCTCCG141150.013.31632770
GTCTTAG26000.013.1979261
GTACTAT19000.012.5316511
TATACCG3952.6628186E-812.4088055
GTATATA37550.012.4020841
TATACTG28350.012.3494025
CTATACT24250.012.2717414
TCTGTAC42050.012.156083
GTACAGG48500.012.1288751
TCTCCGA117850.012.0880470
CGAGCGT10150.012.06735616
Produced by FastQC (version 0.11.5)