FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404384

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404384
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11018212
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCGT4250.017.29812638
GTACTAG12600.017.2279361
GTATTAG24100.016.125681
GTCTTAG24150.015.3674151
GTATATA38100.014.8868271
GTCCTAG26350.014.350111
GTATAGG18350.014.1191231
GCTCTAG35900.013.8486041
GTGCTAG28900.013.6896621
TCAGTAC30950.013.4577433
TAGTGCG5350.013.08471157
GTACTAA20500.012.9799151
GCGATCG5400.012.96596837
GTATTAA31400.012.9342261
GTATAAG21250.012.851321
GTCCTAA22100.012.5154621
GTATTAT29250.012.3288741
GTCTAGA24500.012.2897891
GGTCTAA19500.012.2091771
GTACTAT20250.012.1027791
Produced by FastQC (version 0.11.5)