FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404386

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404386
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11009718
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG13200.018.3015351
GTCTTAG24300.016.4252091
GTATTAG20050.016.2398091
GTCCTAG26500.015.98644351
GTCCTAA21800.015.578571
GTGCTAG26400.014.853421
GGTCTAG21950.014.674581
GTTCTAG31700.014.2476361
GTACTAA20400.014.2449291
CTATACT26000.013.3283254
ATCTCCG140350.013.14197570
TTACGCG1600.00910388213.12471111
GTACATA30150.013.1221051
GCTCTAG29900.012.997631
TCTATAC22650.012.9814983
GTATAGG16600.012.865721
TATACTG31300.012.8607785
GTTCTAA29850.012.6675251
GTATATA36150.012.5906291
GTCCTAT22750.012.465681
Produced by FastQC (version 0.11.5)