FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404388

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404388
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10721498
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG13400.016.7232841
GTATTAG22450.015.5966031
GTTCTAG31000.014.57049751
GGTCTAG19650.014.2552181
GTATAAG20650.014.0735741
GTGCTAG27500.014.005751
GTATTAA30000.013.8890361
GTCTTAA28100.013.7066951
GTCTTAG22100.013.4670671
GTACAGG45700.013.408131
GTTCTAA29650.013.2263421
GTCCTAG27350.013.18641
GTACTAA17900.013.105941
CTAGACT25600.012.9895544
GTATATA36000.012.9358651
CTATACT24650.012.7801574
ATCTCCG140000.012.54947670
GTCCTAA23650.012.4363961
GTACTAT19150.012.4333041
TATACTG29000.012.4322545
Produced by FastQC (version 0.11.5)