FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404389

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404389
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10721498
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG26150.016.5955641
GTATTAG21750.016.5737171
GTGCTAG26500.016.2443081
GTACTAG11100.014.8189311
CTATACT24450.014.4554574
GGTCTAG22800.013.6614611
TTAGACG5150.013.5897694
GTCTTAA28400.013.0625961
GTACTAA19500.012.9223011
GTATATG29350.012.8782721
GTCTTAG22350.012.8403931
GTACATA23100.012.7265091
GTGCTAA22350.012.6838031
GTATTAT30950.012.5517511
GTTCTAG30750.012.5195751
GTCCTAA21250.012.5168951
TCTGACG114100.012.51581370
GTATAGG15800.012.4043181
GTACAGG44450.012.2827281
CTGACGC97300.012.19470870
Produced by FastQC (version 0.11.5)