FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404392

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404392
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12228068
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG28300.017.0444471
GTCCTAA26850.016.4250641
GTATTAA37150.015.766361
GGTCTAA24000.014.6430071
GTACTAA23550.014.0449981
GTCCTAG30300.013.9863341
TCTATAC25400.013.8360253
GTCTTAG29650.013.3633241
GTATATA48250.013.2818011
GTATAGG20300.013.2385341
CTATACT29150.013.1199174
GTACTAG15500.013.11479951
GTCTTAA34100.013.0339691
GTTCTAA37900.012.9089371
GTGCTAG32050.012.9001451
GTTCTAG36650.012.8791711
GTACTAT23650.012.8201441
GGTCTAG23650.012.8201441
CGATCGT4053.430614E-912.7648138
GTCTATG35400.012.7499771
Produced by FastQC (version 0.11.5)