FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404395

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404395
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12307143
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG17650.017.1857831
GTATTAG27200.016.6009831
GTACTAA23700.015.7074891
GTCCTAA26650.015.0034851
GTCCTAG32200.014.9866151
CTATACT30200.014.8317144
GTATATG36150.014.7793311
GTGCTAG31050.014.653581
GGTCTAG26150.014.6312911
GTGCTAA30100.014.54348851
GTATATA49750.014.4805341
GTATTAA36150.014.3025791
GTCTTAG30300.013.7649351
GTATAAG23450.013.6700931
GTACTAT26800.013.6333461
GTGTTAG33200.013.4969811
GGTCTAA24650.013.2842811
GTTCTAG38600.012.948281
GTATTAT38900.012.9370321
TCTATAC26100.012.80503753
Produced by FastQC (version 0.11.5)