FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404396

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404396
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12301847
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG24450.016.7715781
GTATACG4600.016.480511
CTATACT30550.015.2275554
GTCCTAG30950.014.6966761
GTATATA46650.014.25645351
GTGCTAG30400.014.1691011
GTCTTAG28950.014.0455691
GTACTAA23800.014.0443171
GTATTAA36500.013.9725191
GTACTAG15450.013.8283091
TATACTG36150.013.7264515
GTACTAT23100.013.72403051
GTACAAG39750.013.6103211
TCTAGAC28900.013.5929793
GTACATA36300.013.3849991
CCTATAC19700.013.2939783
GTATAAA49200.012.9572391
GTATAGA28850.012.8998231
GTCTTAA34500.012.7848831
GTACAGG53850.012.6702551
Produced by FastQC (version 0.11.5)