FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404397

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404397
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12301847
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG160040.1300942858417927No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG16350.019.1848851
GTATTAG25200.018.0554771
GTGCTAG31200.016.129981
GTCCTAG30000.016.0857891
GTATTAA38650.015.2504331
GTACTAA24300.015.1779491
GGTCTAG26200.014.8666361
GTATATA45850.014.8102531
GTTCTAG37600.014.209521
GTATTAT37100.013.7506551
GTACAGG51500.013.7208871
GTCTTAG27950.013.6891581
GTCTTAA34400.013.6274951
CCTATAC21000.013.4564733
GTATAGG19850.013.3710591
GTATAGA26200.013.2878781
GCTCTAG37100.013.10028651
TCTAGAC28150.013.0991183
GTGTTAG31550.013.0011921
GTCCTAA28650.012.9937571
Produced by FastQC (version 0.11.5)