FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404398

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404398
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13685893
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG19150.017.967581
GTATTAG34200.015.6948411
TATACTG43750.015.1002165
GTCCTAA30400.014.7139131
GTGCTAG35700.014.3607291
GTATATA59200.013.9491571
CTATACT33200.013.8875334
GTATAGG22750.013.7631681
GTCCTAG33600.013.61980151
GTATTAA41600.013.5646611
TTATACT46850.013.5134624
GTCTTAG33300.013.3291931
GCTCTAG39850.013.2105681
GTATTAT45450.013.0212581
GTACTAA26450.013.0086651
GTTCTAG41550.012.8356861
TCTAGAC31850.012.7476753
TCTATAC29650.012.64911653
GTACAGG56150.012.439551
GTCTTAA40750.012.4121811
Produced by FastQC (version 0.11.5)