FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404399

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404399
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13685893
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG19250.018.9862821
GTCCTAG34650.018.5086381
GTATTAG30700.018.1945531
GTACTAA26050.015.8832111
GTTCTAG43250.015.226921
GTATTAA43800.014.6421081
GTCCTAA33500.014.6153191
GTCTTAG32350.014.3887891
CCTATAC22250.013.7745243
GTTCTAA40600.013.6730251
GTCCTAT30250.013.2220081
GGTCTAG28300.013.158371
GTACATA43800.012.9102461
CTATACT33150.012.87813954
GTGCTAG36450.012.7702971
TATACTG43200.012.6674345
GTCTTAA37600.012.6548211
GTGCTAA32700.012.6531381
GTACAAG42350.012.37527751
GTATTAT45450.012.3656941
Produced by FastQC (version 0.11.5)