FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404400

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404400
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13509635
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG16850.017.1566491
GTATTAG29200.015.6755171
GTCCTAG32500.015.3545731
GTACTAA24350.015.1229991
GTCCTAA32800.014.899361
GTATATA55350.014.8604991
GTACTAT25250.013.3572731
GTATAGG22350.013.2426241
GTATTAA41650.013.0555511
GTATAAA55050.013.0034661
GCGCTAG3751.6376362E-812.8484251
GCCCTAA30900.012.8083291
TATACTG40550.012.6461455
GTATACG4651.6007107E-1012.5819841
GTGCTAG34950.012.5057481
GTATATG44200.012.4580721
GTACAGG56650.012.3931971
GTATTGG33500.012.2251811
TACTAGG22050.012.1742982
GTACATA40450.012.1666391
Produced by FastQC (version 0.11.5)