FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005404401

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005404401
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13509635
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG188510.13953744864313508No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG17550.017.4892771
GTATTAG28950.017.1542971
GTCCTAG34350.016.2647441
GTTCTAG39900.014.9531321
GTCCTAA34300.014.5791711
GTCTTAG33200.014.54282951
TCTATAC29250.014.0107173
GTACTAA28150.013.966431
GCTCTAG38050.013.6861481
GTATTAA43650.013.5104811
CTAGACT32650.013.4991044
GTGCTAG37300.013.2216641
GTATAGG22350.012.9616621
GTCTTAA37950.012.9043311
TTATACT46250.012.805454
GTTCTAA44100.012.66879751
TATACTG43900.012.6225345
GTACAGG55200.012.6203451
CTATACT31200.012.4710214
GCCCTAG35400.012.4699761
Produced by FastQC (version 0.11.5)